Analysis of RNA modifications using nanopore sequencing
RNA modifications play a crucial role in many cellular and biological processes, opening up an emerging research field known as epitranscriptomics. To this date, over 150 different RNA modifications have been identified, and only recently has it been possible to map selected RNA modifications at single-nucleotide resolution.
In collaboration with the Helm Group, we synthesize artificial RNA containing various base modifications and analyze them using Oxford Nanopore's direct RNA sequencing approach.
By implementing machine learning algorithms, we want to establish new protocols and tools for the accurate identification of RNA base modifications alongside the nucleotide sequence. This project is in collaboration with Anna Wierczeiko.