Dr. Tamer Butto
Bentzelweg 3, 55128 Mainz
“Deciphering the Epigenetic basis of Resilience”:
Chronic stressor exposure can have varying impact upon long-term behavioral phenotypes. While some individuals develop stress-induced mental dysfunctions and mood related disorders such as persevering anxiety, others are resilient. In this research project, we focus on the effects of chronic stress on distinct epigenetic mechanisms altered in the brain during the stress-response and more specifically on activated neuronal populations. We use chronic social defeat (CSD) to classify the stress-susceptible and resilient mice. Following nuclei isolation of the activated neuronal population, we apply nuclear RNA-seq, ATAC-seq and RRBS to identify the transcriptomic and epigenetic changes occurring the activated neuronal population of each behavioral group. Our aim is to shed light into the molecular mechanisms associated with resilient behavior which could be translated into future studies to prevent stress-related dysfunctions in humans.
This project is part of CRC1193 Resilience - Project A05 where we work in collaboration with Dr. Jennifer Winter (Uni-Med, Mainz).
“Uncovering the relationship between gut microbiota, miRNA regulation and their role in resilience”:
In this project, we are interested in the “communication” between the gut microbiota and the host, particularly following stress exposure. The microbiota consists of all microbial communities inhabiting the gut which produce and secrete a wide variety of metabolites, small molecules, and endogenous compounds that ultimately influence the host cellular activity. In this research project, we explore the possible communication mechanisms focusing particularly on small non-coding RNA (specifically miRNAs), to identify a dynamic communication network that could be altered following stress exposure and potential stress adaptation mechanisms. This project is a collaboration with Dr. Kristina Endres (Uni-Med Mainz).
Martin Schüle , Tamer Butto , Sri Dewi , Susanne Strand , Susanne Gerber , Kristina Endres , Susann Schweiger , Jennifer Winter. mTOR inhibition in primary neurons and the developing brain represses transcription of cholesterol biosynthesis genes and alters cholesterol levels. bioRxiv, DOI https://doi.org/10.1101/2020.09.04.282772 (2020)